{"id":2537,"date":"2010-03-08T23:02:59","date_gmt":"2010-03-08T14:02:59","guid":{"rendered":"http:\/\/yasu2.prosou.nu\/blog\/index.php\/2010\/03\/08\/sgmj_2010_day_2\/"},"modified":"2010-03-08T23:02:59","modified_gmt":"2010-03-08T14:02:59","slug":"sgmj_2010_day_2","status":"publish","type":"post","link":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/2010\/03\/08\/2537\/","title":{"rendered":"SGMJ 2010: Day 2"},"content":{"rendered":"<p>[\u9ad8\u6e29\u30fb\u9ad8\u5727\u306e\u5730\u4e0b\u539f\u6cb9\u9271\u5e8a\u306b\u304a\u3051\u308b\u56fa\u6709\u5fae\u751f\u7269\u7fa4\u3068\u305d\u306e\u7279\u6027]<br \/>\n\u79cb\u7530\u5e02\u516b\u6a4b\u3001\u65b0\u516b\u6a4b\u6cb9\u7530\u3002<br \/>\n&#8211; SR123 1687m, 98\u5ea6, 113atm<br \/>\n&#8211; SR39 1100m, 74\u5ea6, 29.2atm<br \/>\n\u306e\u3075\u305f\u3064\u306e\u4e95\u6238\u304b\u3089\u3001\u5730\u5c64\u6c34\u3092\u542b\u3081\u3066\u30b5\u30f3\u30d7\u30eb\u3092\u76f4\u63a5\u63a1\u53d6\u3002\u3053\u3053\u306f\u6d77\u6c34\u6ce8\u5165\u3068\u304b\u305d\u3046\u3044\u3046\u3053\u3068\u306f\u3057\u3066\u3044\u306a\u3044\u4e95\u6238\u3067\u3001\u5730\u8868\u306e\u53d6\u308a\u51fa\u3057\u53e3\u3058\u3083\u306a\u304f\u3066\u305d\u306e\u5730\u5c64\u306e\u3068\u3053\u308d\u304b\u3089\u76f4\u63a5\u30b5\u30f3\u30d7\u30eb\u3092\u63a1\u53d6\u3057\u3066\u3044\u308b\u3002<br \/>\n\u83cc\u7a2e\u306e\u5206\u5e03\u306b\u306f\u9055\u3044\u304c\u3042\u308b\u3002<br \/>\n\u9ad8\u71b1\u6027\u306e\u83cc\u306f GC-content \u304c\u9ad8\u3044\u306e\u3067\u3001\u305d\u308c\u3092\u4f7f\u3063\u3066\u4e26\u3079\u66ff\u3048\u308b\u3068\u9762\u767d\u3044\u3002\u5730\u8868\u306e\u53d6\u308a\u51fa\u3057\u53e3\u3067\u306f\u3044\u308d\u3093\u306a\u83cc\u304c\u5897\u6b96\u3057\u3066\u3044\u308b\u3002\u6cb9\u4e95\u3092\u6398\u308b\u3068\u304d\u306b\u5ca9\u3092\u524a\u308b\u306e\u306b\u305f\u304f\u3055\u3093\u6c34\u3092\u6d41\u3059\u306e\u3067\u3001\u305d\u3053\u3067\u30b3\u30f3\u30bf\u30df\u3057\u3066\u3001\u6614\u306f\u3044\u306a\u304b\u3063\u305f\u9023\u4e2d\u304c\u3044\u308b\u53ef\u80fd\u6027\u3082\u3042\u308b\u3002<br \/>\n\u91cd\u8981\u306a\u306e\u306f Bacteria \u3068 Archaea \u304c\u3046\u307e\u304f\u307e\u3058\u3063\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u3002\u9178\u7d20\u304c\u306a\u3044\u72b6\u6cc1\u3067 Bacteria \u304c\u6709\u6a5f\u7269\u304b\u3089\u6c34\u7d20\u3092\u751f\u6210\u3057\u3066 Archaea \u304c\u305d\u3053\u304b\u3089\u30e1\u30bf\u30f3\u3092\u4f5c\u308b\u3053\u3068\u306b\u3088\u308b\u6804\u990a\u5171\u751f\u3002<br \/>\n[\u30e9\u30f3\u30c1\u30e7\u30f3\u30bb\u30df\u30ca\u30fc]<br \/>\nRoche GS Junior<br \/>\n&#8211; Read length: 350bp<br \/>\n&#8211; Reads\/run 100,000<br \/>\n&#8211; \u30ec\u30fc\u30b6\u30fc\u30d7\u30ea\u30f3\u30bf\u3068\u540c\u3058\u304f\u3089\u3044\u306e\u30b5\u30a4\u30ba\u3067\u3001\u673a\u306b\u304a\u3051\u308b\u3002\u7d4c\u6e08\u6027\u3092\u8ffd\u6c42\u3002<br \/>\n&#8211; 454 \u306e 1\/3 \u304f\u3089\u3044\u306e\u5024\u6bb5 (\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u3068\u540c\u3058\u304f\u3089\u3044\u306e\u5024\u6bb5?)<br \/>\n&#8211; 2000\u4e07\u5186\u3042\u308c\u3070\u5468\u8fba\u6a5f\u5668\u3082\u5168\u90e8\u8cb7\u3048\u307e\u3059\u3002\u672c\u4f53\u306f1500\u4e07\u5186\u304f\u3089\u3044\u304b\u306a? \u30b3\u30f3\u30d4\u30e5\u30fc\u30bf\u3082\u4ed8\u5c5e<br \/>\n&#8211; 1 run \u306e\u8cbb\u7528\u306f10\u4e07\u5186\u304f\u3089\u3044<br \/>\nGS FLX Titanium 1k<br \/>\n&#8211; Read length: 1000bp<br \/>\n&#8211; Reads\/run: 1,000,000<br \/>\n\u30ac\u30b9\u30b1\u30c3\u30c8\u3067\u4ed5\u5207\u308b\u306e\u3068 tag \u3092\u4f7f\u3063\u3066\u5206\u3051\u308b\u306e\u3068\u3069\u3063\u3061\u304c\u3044\u3044\uff1f \u2192 \u30b5\u30f3\u30d7\u30eb\u304c\u5c11\u306a\u3044\u3068\u304d\u306f\u30ac\u30b9\u30b1\u30c3\u30c8\u4f7f\u3063\u305f\u65b9\u304c\u7c21\u5358\u3002<br \/>\n[\u53d7\u8cde\u8a18\u5ff5\u8b1b\u6f14]<br \/>\n\u771f\u6838\u751f\u7269\u306e\u7814\u7a76\u306f\u9175\u6bcd\u3084\u52d5\u7269\u7d30\u80de\u3067\u884c\u308f\u308c\u3066\u304a\u308a\u3001\u690d\u7269\u3067\u306f\u9045\u308c\u3066\u3044\u308b\u3002<br \/>\n\u690d\u7269\u306f\u500d\u6570\u4f53\u304c\u591a\u3044\u306e\u3067\u3057\u3093\u3069\u3044\u304c\u3001\u5149\u5408\u6210\u3092\u3059\u308b\u771f\u6838\u5fae\u751f\u7269\u3067\u3042\u308c\u3070\u3001\u3044\u308d\u3044\u308d\u306a\u3053\u3068\u304c\u308f\u304b\u308b\u3002<br \/>\n\u306a\u308b\u307b\u3069\u306a\u30fc\u3002<br \/>\n\u30a2\u30d6\u30e9\u30e0\u30b7\u306e\u5171\u751f\u7d30\u83cc\u304b\u3089\u306e\u6c34\u5e73\u8ee2\u79fb\u907a\u4f1d\u5b50\u7fa4\u3002\u7d30\u80de\u5185\u5171\u751f\u3002<br \/>\n\u3082\u3046\u7d30\u83cc\u306f\u4f4f\u3093\u3067\u3044\u306a\u3044\u3051\u3069\u3001\u6c34\u5e73\u8ee2\u79fb\u3057\u3066\u6b8b\u3063\u3066\u3044\u308b\u3001\u3068\u3044\u3046\u306e\u304c\u3042\u308b\u3002<br \/>\n\u5171\u751f\u3068\u304b\u5bc4\u751f\u306f\u9762\u767d\u3044\u306a\u3042 (\u5bc4\u751f\u3055\u308c\u308b\u306e\u306f\u3001\u3044\u3084\u3060\u3051\u3069)\u3002<br \/>\n[\u30de\u30eb\u30ab\u30e1\u30e0\u30b7\u985e\u306e\u8178\u5185\u306b\u5b58\u5728\u3059\u308b\u5fc5\u9808\u5171\u751f\u7d30\u83cc\u30a4\u30b7\u30ab\u30ef\u30a8\u30e9\u306e\u6bd4\u8f03\u30b2\u30ce\u30e0\u89e3\u6790]<br \/>\n\u690d\u7269\u5e2b\u7ba1\u6db2\u3092\u4e3b\u305f\u308b\u6804\u990a\u6e90\u3068\u3057\u3001\u8178\u5185\u306b\u5fc5\u9808\u5171\u751f\u7d30\u83cc\u30a4\u30b7\u30ab\u30ef\u30a8\u30e9\u3092\u3082\u3064\u3002<br \/>\n\u30c0\u30a4\u30ba\u3067\u3069\u308c\u304f\u3089\u3044\u7e41\u6b96\u3067\u304d\u308b\u304b\u3001\u3068\u3044\u3046\u306e\u304c\u5171\u751f\u7d30\u83cc\u3067\u6c7a\u307e\u3063\u3066\u3044\u308b\u3002<br \/>\n[Metagenomics-based 16S ribosomal RNA gene profiling]<br \/>\n454\u3092\u4f7f\u3046\u306e\u3067\u3001chimera \u304c\u3067\u304d\u305f\u308a PCR bias \u304c\u304b\u304b\u3063\u305f\u308a\u3057\u306a\u3044\u3002<br \/>\n\u305d\u3046\u304b\u30fc\u3002\u3046\u30fc\u3093\u3002\u3084\u3063\u3071\u308a metagenomics \u3063\u3066\u96e3\u3057\u3044\u3093\u3060\u306a\u3042\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>[\u9ad8\u6e29\u30fb\u9ad8\u5727\u306e\u5730\u4e0b\u539f\u6cb9\u9271\u5e8a\u306b\u304a\u3051\u308b\u56fa\u6709\u5fae\u751f\u7269\u7fa4\u3068\u305d\u306e\u7279\u6027] \u79cb\u7530\u5e02\u516b\u6a4b\u3001\u65b0\u516b\u6a4b\u6cb9\u7530\u3002 &#8211; SR123 1687m, 98\u5ea6, 113atm &#8211; SR39 1100m, 74\u5ea6, 29.2atm \u306e &hellip; <a href=\"https:\/\/yasu2.prosou.nu\/blog\/index.php\/2010\/03\/08\/2537\/\" class=\"more-link\"><span class=\"screen-reader-text\">&#8220;SGMJ 2010: Day 2&#8221; \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"activitypub_content_warning":"","activitypub_content_visibility":"","activitypub_max_image_attachments":4,"activitypub_interaction_policy_quote":"anyone","activitypub_status":"","footnotes":""},"categories":[10],"tags":[],"class_list":["post-2537","post","type-post","status-publish","format-standard","hentry","category-conference-logs"],"_links":{"self":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2537","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/comments?post=2537"}],"version-history":[{"count":0,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2537\/revisions"}],"wp:attachment":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/media?parent=2537"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/categories?post=2537"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/tags?post=2537"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}