{"id":2282,"date":"2009-03-07T21:47:14","date_gmt":"2009-03-07T12:47:14","guid":{"rendered":"http:\/\/yasu2.prosou.nu\/blog\/index.php\/2009\/03\/07\/sgmj_day_2_and_3\/"},"modified":"2009-03-07T21:47:14","modified_gmt":"2009-03-07T12:47:14","slug":"sgmj_day_2_and_3","status":"publish","type":"post","link":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/2009\/03\/07\/2282\/","title":{"rendered":"SGMJ Day 2 and 3"},"content":{"rendered":"<p>[ \u539f\u59cb\u7684\u306a\u771f\u6838\u85fb\u985e Cyanophora paradoxa \u306e\u30d5\u30a3\u30b3\u30d3\u30ea\u30bd\u30fc\u30e0\u306e\u89e3\u6790 ]<br \/>\n\u304a\u305d\u3089\u304f\u3082\u3063\u3068\u3082\u539f\u59cb\u7684\u306a\u771f\u6838\u85fb\u985e\u3002<br \/>\n\u8449\u7dd1\u4f53\u306e2\u679a\u306e\u819c\u306e\u9593\u306b\u30da\u30d7\u30c1\u30c9\u30b0\u30ea\u30ab\u30f3\u3092\u6301\u3064\u3002<br \/>\n\u5f62\u6210\u306e\u3055\u308c\u304b\u305f\u3082\u307b\u304b\u306e\u751f\u7269\u306e\u8449\u7dd1\u4f53\u3068\u306f\u3060\u3044\u3076\u9055\u3046\u3002<br \/>\n[ \u690d\u7269\u5185\u751f\u7d30\u83cc\u3000Azospirillum sp. B510 \u306e\u30b2\u30ce\u30e0\u69cb\u9020\u89e3\u6790 ]<br \/>\nrrn \u306e\u30b3\u30d4\u30fc\u304c\u305f\u304f\u3055\u3093\u30029\u30bb\u30c3\u30c8\u3002<br \/>\n\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u306e\u307f\u5b58\u5728\u3057\u3066\u3044\u308b\u3082\u306e\u3082\u3042\u308b\u30fb\u30fb\u30fb\u5fc5\u9808\u907a\u4f1d\u5b50\u304c\u4e3b\u67d3\u8272\u4f53\u306b\u306a\u3044\u3001\u3068\u3044\u3046\u3053\u3068\u306f\u3042\u308b\u306e\u304b\uff1f<br \/>\nE.coli \u306e\u5fc5\u9808\u907a\u4f1d\u5b50 303 \u306e\u3046\u3061\u300118\u907a\u4f1d\u5b50\u304c\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u914d\u5217\u0005\u306b\u30d2\u30c3\u30c8\u3057\u305f!!<br \/>\n\u8907\u6570\u306e\u67d3\u8272\u4f53\u69d8\u69cb\u9020\u3092\u6301\u3064\u30b1\u30fc\u30b9\u306f\u3044\u304f\u3064\u304b\u306e proteobacteria \u3067\u3082\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u304c\u30016 \u3064\u3082\u3042\u308b\u306e\u306f\u73cd\u3057\u3044\u3002<br \/>\n[ \u690d\u7269\u751f\u606f\u5fae\u751f\u7269\u306e\u7fa4\u8846\u69cb\u9020\u306b\u5bfe\u3059\u308b\u6839\u7c92\u5171\u751f\u7cfb\u306e\u5f71\u97ff ]<br \/>\n\u904e\u5270\u7a92\u7d20\u65bd\u80a5\u3067\u6839\u7c92\u83cc\u306e\u30d0\u30e9\u30f3\u30b9\u304c\u5909\u308f\u308b\u3002\u306a\u308b\u307b\u3069\u30fc\u3002<br \/>\n[ \u6539\u826f\u578b\u6c4e\u7528 RIVET (Recombinant in vivo expression technology) \u306b\u3088\u308b\u6839\u7c92\u83cc\u5171\u751f\u907a\u4f1d\u5b50\u306e\u65b0\u898f\u691c\u51fa ]<br \/>\n\u6839\u7c92\u83cc\u306f\u6839\u6bdb\u304b\u3089\u611f\u67d3\u3002\u5bbf\u4e3b\u7279\u7570\u6027\u304c\u3042\u308b\u3002<br \/>\n\u5171\u751f\u306b\u306f\u52b9\u304b\u306a\u3044\u3051\u3069\u7af6\u5408\u306b\u52b9\u304f\u907a\u4f1d\u5b50\u304c\u3042\u308b? \u3075\u3046\u3080\u3001\u304a\u3082\u3057\u308d\u3044\u306a\u3002<br \/>\n[ \u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u3092\u7528\u3044\u305f\u611f\u67d3\u75c7\u306e\u30e1\u30bf\u30b2\u30ce\u30df\u30c3\u30af\u8a3a\u65ad ]<br \/>\n\u60a3\u8005\u3055\u3093\u304b\u3089\u306e\u81e8\u5e8a\u691c\u4f53\u3092\u76f4\u63a5\u30b7\u30fc\u30b1\u30f3\u30b9\u3057\u3066\u8a3a\u65ad\u3059\u308b\u8a66\u307f\u3002<br \/>\n\u5f93\u6765\u306e\u7d30\u83cc\u691c\u67fb\u3067\u306f\u57f9\u5730\u3084 PCR \u30d7\u30e9\u30a4\u30de\u30fc\u3092\u3044\u308d\u3044\u308d\u7528\u610f\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u304b\u3063\u305f\u304c\u3001\u30b7\u30fc\u30b1\u30f3\u30b5\u3092\u7528\u3044\u308c\u3070\u5358\u4e00\u306e\u624b\u6cd5\u3067\u691c\u67fb\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u3002\u30a6\u30a4\u30eb\u30b9\u3084\u5bc4\u751f\u866b\u306b\u3082\u9069\u7528\u53ef\u80fd\u304b\u3082\u3057\u308c\u306a\u3044\u3002\u305f\u3060\u3057\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u306a\u3069\u306e\u5834\u5408\u3001RNA \u62bd\u51fa\uff0b cDNA \u306e\u4f5c\u6210\u3001\u304c\u5fc5\u8981\u3002<br \/>\n[ \u30bf\u30a4\u30ea\u30f3\u30b0\u30a2\u30ec\u30a4\u306b\u3088\u308b\u3089\u3044\u83cc\u30b2\u30ce\u30e0\u5168\u57df\u306e\u7db2\u7f85\u7684\u767a\u73fe\u89e3\u6790 ]<br \/>\nRNA \u767a\u73fe\u3092 RT-PCR \u3067\u5b9a\u91cf\u3057\u3066\u3001\u3044\u304f\u3064\u304b\u306e\u507d\u907a\u4f1d\u5b50\u304c\u8ee2\u5199\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<br \/>\n\u30bf\u30a4\u30ea\u30f3\u30b0\u30a2\u30ec\u30a4\u306f\u305d\u308c\u304b\u3089\u3002<br \/>\n60bp \u306e\u30d7\u30ed\u30fc\u30d6\u3092 18bp \u9593\u9694\u3002\u76f8\u88dc\u9396\u5206\u3082\u4f5c\u6210\u3002<br \/>\n\u6cbb\u7642\u524d\u5f8c\u306e RNA \u767a\u73fe\u30d1\u30bf\u30fc\u30f3\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u3068\u75c5\u614b\u89e3\u6790\u304c\u3067\u304d\u305f\u308a\u3059\u308b\u304b\u306a\u30fc\u3002<br \/>\n\u507d\u907a\u4f1d\u5b50\u304c\u306a\u3093\u3089\u304b\u306e\u5f79\u5272\u3092\u3082\u3063\u3066\u3044\u3066\u3001\u9078\u629e\u5727\u306b\u5bfe\u3059\u308b\u62b5\u6297\u529b\u306b\u306a\u3063\u3066\u3044\u308b\uff1f<br \/>\nM.ulcerans \u3082\u3051\u3063\u3053\u3046\u507d\u907a\u4f1d\u5b50\u304c\u3042\u308b\u306e\u3067\u3001\u507d\u907a\u4f1d\u5b50\u304c\u6b8b\u308b\u3001\u3068\u3044\u3046\u306e\u306f Mycobacterium \u306b\u7279\u6709\u3060\u3063\u305f\u308a\u3059\u308b\u306e\u3067\u306f\uff1f (\u307e\u3060\u691c\u8a0e\u3055\u308c\u3066\u306a\u3044\u305d\u3046\u3067\u3059\u304c)<br \/>\n[SOLiD]<br \/>\ncsfasta format \u3068\u3044\u3046\u306e\u3067\u914d\u5217\u304c\u3067\u3066\u304f\u308b\u30022base encoding.<br \/>\n[ Structure evaluation using the consensus and quality assessment for protein modeling ]<br \/>\n\u69cb\u9020\u4e88\u6e2c\u30b3\u30f3\u30c6\u30b9\u30c8\u306e\u8a71\u3002<br \/>\n[ BL-SOM ]<br \/>\n\u6c60\u6751\u5148\u751f\u306e\u3068\u3053\u308d\u306e\u767a\u8868\u3002<br \/>\n\u751f\u7269\u7a2e\u306e\u63a8\u5b9a\u3060\u3051\u3067\u306f\u306a\u304f\u3066\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u6a5f\u80fd\u63a8\u5b9a\u306b\u3082\u4f7f\u3048\u308b?<br \/>\nSOM \u306e specificity \/ sensitivity \u306f\u3069\u3046\u3084\u3063\u3066\u8a55\u4fa1\u3059\u308b\u306e? \u2192 \u305f\u3068\u3048\u3070 COG \u3092\u6b63\u89e3\u30bb\u30c3\u30c8\u306b\u3057\u3066\u8a55\u4fa1\u3059\u308b\u3068\u304b\u3002<br \/>\n[ Genome Matcher ]<br \/>\n\u5927\u576a\u3055\u3093\u3002<br \/>\n\u914d\u5217\u89e3\u6790\u306e\u3068\u3053\u308d\u306f BLAST \u3068 MUMmer.<br \/>\n\u591a\u5bfe\u591a\u306e dot-plot \u306e\u3084\u308a\u304b\u305f\u306f\u53c2\u8003\u306b\u306a\u308b\u304b\u3082? \u3067\u3082\u304a\u4e92\u3044\u3042\u3093\u307e\u308a\u30de\u30cd\u3057\u306a\u3044\u65b9\u304c\u3044\u3044\u3088\u306d&#8230;<br \/>\nExcel2Fasta \u3068\u304b\u3001GenBank2Excel \u3068\u304b\u3001\u3044\u3051\u3066\u308b\u3002<br \/>\nblastn \u3068\u304b tblastn \u306e\u3068\u3053\u308d\u3092\u5165\u308c\u66ff\u3048\u308b\u306e\u306f\u30e6\u30fc\u30b6\u304c\u3067\u304d\u308b\uff1f\u2192\u30d7\u30ed\u30b0\u30e9\u30e0\u306a\u304a\u3055\u306a\u3042\u304b\u3093\u3002<br \/>\n\u3044\u308d\u3093\u306a\u30c4\u30fc\u30eb\u306f pipeline \u306b\u3067\u304d\u308b? \u2192 Excel \u306b\u8cbc\u308a\u4ed8\u3051\u3066\u304f\u308a\u304b\u3048\u3057\u3002<br \/>\n[ \u5927\u91cf\u306e\u30aa\u30fc\u30bd\u30ed\u30b0\u30c7\u30fc\u30bf\u3092\u7528\u3044\u305f\u5fae\u751f\u7269\u306e\u7cfb\u7d71\u63a8\u5b9a ]<br \/>\n\u7740\u76ee\u3059\u308b\u907a\u4f1d\u5b50\u3092\u304b\u3048\u308b\u3068\u3001\u7a2e\u306e\u7cfb\u7d71\u95a2\u4fc2\u304c\u5909\u308f\u3063\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308b\u3002<br \/>\n\u539f\u6838\u751f\u726917\u9580\u306b\u3064\u3044\u3066\u65b0\u3057\u3044\u65b9\u6cd5\u3067\u30aa\u30fc\u30bd\u30ed\u30b0\u30c7\u30fc\u30bf\u30bb\u30c3\u30c8\u3092\u4f5c\u6210\u3057\u3001\u305d\u308c\u3092\u3082\u3068\u306b\u7cfb\u7d71\u95a2\u4fc2\u3092\u6c7a\u5b9a\u3002\u65b0\u3057\u3044\u7cfb\u7d71\u6a39\u5206\u5c90\u70b9\u8a55\u4fa1\u6cd5\u3092\u958b\u767a\u3059\u308b\u3002<br \/>\nMBGD\u304b\u3089\u5165\u624b\u3057\u305f\u30aa\u30fc\u30bd\u30ed\u30b0\u30bb\u30c3\u30c8\u304b\u3089\u3001\u30a2\u30a6\u30c8\u30d1\u30e9\u30ed\u30b0(\u7a2e\u5206\u5316\u306e\u524d\u306b\u8d77\u3053\u3063\u305f\u907a\u4f1d\u5b50\u91cd\u8907\u306b\u3088\u3063\u3066\u751f\u3058\u305f\u907a\u4f1d\u5b50\u7fa4)\u3092\u9664\u53bb\u3002\u305d\u308c\u3092\u3082\u3068\u306b\u9023\u7d50\u7cfb\u7d71\u6a39\u3092\u4f5c\u6210\u3057\u3001\u9023\u7d50\u7cfb\u7d71\u6a39\u306e\u305d\u308c\u305e\u308c\u306e\u5206\u5c90\u70b9\u304c\u3001\u5404\u30aa\u30fc\u30bd\u30ed\u30b0\u7cfb\u7d71\u6a39\u306b\u3088\u3063\u3066\u3069\u308c\u3060\u3051\u652f\u6301\u3055\u308c\u3066\u3044\u308b\u304b\u3092\u8a08\u7b97\u3059\u308b\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>[ \u539f\u59cb\u7684\u306a\u771f\u6838\u85fb\u985e Cyanophora paradoxa \u306e\u30d5\u30a3\u30b3\u30d3\u30ea\u30bd\u30fc\u30e0\u306e\u89e3\u6790 ] \u304a\u305d\u3089\u304f\u3082\u3063\u3068\u3082\u539f\u59cb\u7684\u306a\u771f\u6838\u85fb\u985e\u3002 \u8449\u7dd1\u4f53\u306e2\u679a\u306e\u819c\u306e\u9593\u306b\u30da\u30d7\u30c1\u30c9\u30b0\u30ea\u30ab\u30f3\u3092\u6301\u3064\u3002 \u5f62\u6210\u306e\u3055\u308c\u304b\u305f\u3082\u307b\u304b\u306e\u751f\u7269\u306e\u8449\u7dd1\u4f53\u3068\u306f\u3060\u3044\u3076 &hellip; <a href=\"https:\/\/yasu2.prosou.nu\/blog\/index.php\/2009\/03\/07\/2282\/\" class=\"more-link\"><span class=\"screen-reader-text\">&#8220;SGMJ Day 2 and 3&#8221; \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"activitypub_content_warning":"","activitypub_content_visibility":"","activitypub_max_image_attachments":4,"activitypub_interaction_policy_quote":"anyone","activitypub_status":"","footnotes":""},"categories":[10,4],"tags":[],"class_list":["post-2282","post","type-post","status-publish","format-standard","hentry","category-conference-logs","category-4"],"_links":{"self":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2282","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/comments?post=2282"}],"version-history":[{"count":0,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2282\/revisions"}],"wp:attachment":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/media?parent=2282"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/categories?post=2282"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/tags?post=2282"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}