\u53bb\u5e74\u50d5\u304c\u5199\u771f\u3092\u64ae\u308a\u307e\u304f\u3063\u3066\u3044\u305f\u304b\u3089\u304b (\u7b11)\u3001\u64ae\u5f71\u3057\u3061\u3083\u3060\u3081\u3088\u3001\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u3063\u3066\u3044\u305f\u306e\u3067\u3001\u4eca\u5e74\u306f\u6587\u7ae0\u3060\u3051\u3002
\n\u4eca\u65e5\u304b\u3089\u4e2d\u592e\u5927 @ \u5f8c\u697d\u5712 \u3067\u3084\u3063\u3066\u307e\u3059\u3002
\n\u521d\u65e5\u306e\u4eca\u65e5\u306f\u3001\u5927\u5b66\u3067\u3042\u308c\u3053\u308c\u3084\u3063\u3066\u305f\u306e\u3067\u3001\u5915\u65b9\u306e\u30dd\u30b9\u30bf\u30fc\u30bb\u30c3\u30b7\u30e7\u30f3\u304b\u3089\u3002
\n[ \u6d77\u6d0b\u690d\u7269\u30d7\u30e9\u30f3\u30af\u30c8\u30f3\u306b\u611f\u67d3\u3059\u308b\u30a6\u30a4\u30eb\u30b9\u306e\u30b2\u30ce\u30e0\u6027\u72b6 ]
\n\u65e2\u77e5\u306e\u30a6\u30a4\u30eb\u30b9\u30d5\u30a1\u30df\u30ea\u306b\u5c5e\u3055\u306a\u3044\u3082\u306e\u3002
\ndsDNA, ssDNA, ssRNA (ds=double strand, ss=single strand) \u30a6\u30a4\u30eb\u30b9\u3092\u5206\u96e2\u3002
\n\u4e00\u672c\u9396\u3067\u3001\u4e00\u90e8\u306b\u3060\u3051\u4e8c\u672c\u9396\u9818\u57df\u304c\u3042\u308b DNA \u3068\u304b\u3002\u4e0d\u601d\u8b70\u3061\u3083\u3093\u3002
\n\u30a4\u30f3\u30c6\u30a4\u30f3 = \u524d\u99c6\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u304b\u3089 splice \u3055\u308c\u308b\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u7248\u306e\u30a4\u30f3\u30c8\u30ed\u30f3\u307f\u305f\u3044\u306a\u3082\u306e\u3002
\npartial double strand DNA \u3068\u3044\u3046\u306e\u306f\u307b\u304b\u306b\u3082\u3042\u308b\u3089\u3057\u3044\u3002
\n\u5bbf\u4e3b\u306f\u85fb\u985e\u3068\u304b\u3089\u3057\u3044\u3002
\n[ Acytostelium \u5c5e\u7d30\u80de\u6027\u7c98\u83cc\u306e\u30b2\u30ce\u30e0\u30fbcDNA \u89e3\u6790 ]
\nD. discoidium A. Acytostelium
\nsize 36M \/ 28M
\nAT contents 78.8% \/ 43.7%
\nCoverage 8x \/ 6x
\nGene model 13,340 \/ 11,xxx
\nAcytostelium \u306e\u907a\u4f1d\u5b50\u69cb\u9020\u3092\u78ba\u5b9a\u3057\u3066\u3001\u304a\u304a\u3088\u305d\u306e\u767a\u73fe\u60c5\u5831\u3092\u5f97\u305f\u3044\u3002
\ncDNA \u3092\u4f5c\u3063\u3066\u30a2\u30bb\u30f3\u30d6\u30eb\u3057\u3066\u3001\u30b2\u30ce\u30e0\u306b\u5f53\u3066\u305f\u3089 3,451 \u500b\u5f53\u305f\u3063\u305f\u3002
\n[ \u30b2\u30ce\u30e0\u89e3\u6790\u304c\u660e\u3089\u304b\u306b\u3057\u305f\u30b7\u30ed\u30a2\u30ea\u8178\u5185\u539f\u751f\u751f\u7269\u7d30\u80de\u5185\u5171\u751f\u7d30\u83cc\u306e\u7a7a\u4e2d\u7a92\u7d20\u56fa\u5b9a\u80fd ]
\n\u6728\u6750\u306b\u306f\u7a92\u7d20\u304c\u307b\u3068\u3093\u3069\u306a\u3044\u304b\u3089\u3001\u305d\u308c\u3060\u3051\u98df\u3079\u3066\u3082\u751f\u304d\u3066\u3044\u3051\u308b\u308f\u3051\u304c\u306a\u304f\u3066\u3001\u8178\u5185\u7d30\u83cc\u304c\u7a7a\u4e2d\u306e\u7a92\u7d20\u3092\u56fa\u5b9a\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u30021uL \u304f\u3089\u3044\u306e\u8178\u306b\u3001\u6570\u5343\u306e\u539f\u751f\u751f\u7269\u3068\u4e00\u5104\u306e\u8178\u5185\u7d30\u83cc\u3002
\n\u539f\u751f\u751f\u7269\u306f\u6728\u7247\u3092\u98df\u3079\u3066\u3044\u308b\u3002
\n\u5171\u751f\u7d30\u83cc\u306f\u57f9\u990a\u3067\u304d\u306a\u3044\u306e\u3067\u3001\uff11\u7d30\u80de\u3092\u53d6\u308a\u51fa\u3057\u3066\u304d\u3066 DNA \u3092\u5897\u5e45\u3057\u3066\u914d\u5217\u8aad\u3093\u3060\u3002
\n\u7a92\u7d20\u56fa\u5b9a\u907a\u4f1d\u5b50\u3092\u306f\u3058\u3081\u3068\u3057\u3066\u3001\u591a\u304f\u306e\u907a\u4f1d\u5b50\u304c Bacteroidales \u306e\u3082\u306e\u306b\u4f3c\u3066\u3044\u308b\u3002\u6c34\u5e73\u4f1d\u642c?
\n\u6728\u7247\u3092\u539f\u751f\u751f\u7269\u304c\u5206\u89e3\u3057\u3066\u5358\u7cd6\u3092\u3064\u304f\u308b\u3068\u3001\u305d\u306e\u4e00\u90e8\u3092\u5171\u751f\u7d30\u83cc\u304c\u3068\u308a\u3053\u3093\u3067\u3001\u7a92\u7d20\u4ee3\u8b1d\u306b\u4f7f\u3063\u3066\u3044\u308b\u3002\u3059\u3052\u30fc\u306a\u3002
\n[ \u6a5f\u80fd\u6027\u30e1\u30bf\u30b2\u30ce\u30e0\u89e3\u6790\u306b\u3088\u308a\u660e\u3089\u304b\u306b\u3055\u308c\u305f\u74b0\u5883\u4e2d\u306e\u82b3\u9999\u74b0\u5206\u89e3\u907a\u4f1d\u5b50\u306e\u5b9f\u614b ]
\n\u5206\u89e3\u30e1\u30ac\u30d7\u30e9\u30b9\u30df\u30c9 (50kb \u4ee5\u4e0a) \u304c\u3042\u3063\u3066(\u67d3\u8272\u4f53\u306b\u4e57\u3063\u3066\u3044\u308b\u3053\u3068\u3082\u3042\u308b\u3051\u3069) \u3001\u5206\u89e3\u907a\u4f1d\u5b50\u7fa4\u306f\u3060\u3044\u305f\u3044\u3072\u3068\u307e\u3068\u307e\u308a\u306e\u30aa\u30da\u30ed\u30f3\u69cb\u9020\u306b\u306a\u3063\u3066\u3044\u308b\u3002
\n[ \u74b0\u72b6DNA\u5408\u6210\u6cd5\u304a\u3088\u3073cell-free cloning\u6cd5 ]
\n\u5fae\u91cf\u306e\u5fae\u751f\u7269 DNA \u5897\u5e45\u306e\u5834\u5408\u3001\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u304c\u554f\u984c\u3067\u3001\u65e2\u77e5\u914d\u5217\u3058\u3083\u306a\u3044\u3068\u3060\u3081\u3002
\nbyproduct = DNA \u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f7f\u3046\u306e\u304c\u554f\u984c\u3067\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e single strand \u306b\u304f\u3063\u3064\u3044\u3061\u3083\u3046\u3002
\nRNA \u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f7f\u3048\u3070\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u7531\u6765\u306e byproduct \u304c\u306a\u304f\u306a\u308b\u3002
\n[ Pre-amplified inverse PCR \u3092\u7528\u3044\u305f\u74b0\u5883\u8a66\u6599\u304b\u3089\u306e\u65b0\u898f\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u306e\u53d6\u5f97 ]
\n\u907a\u4f1d\u5b50\u306e\u5168\u9577\u3092\u5fa9\u5143\u3059\u308b Inverse PCR \u306f (\u305f\u3076\u3093 self ligation \u306e\u52b9\u7387\u304c\u60aa\u3044\u306e\u3067) PCR \u3088\u308a\u305f\u304f\u3055\u3093\u8a66\u6599\u304c\u5fc5\u8981\u3002
\n\u4e8b\u524d\u306b\u5bfe\u8c61\u306e\u3068\u3053\u308d\u3060\u3051\u5897\u5e45\u3057\u305f\u3044\u3002
\n\u7d42\u308f\u3063\u305f\u3042\u3068\u306f\u3001\u306a\u304b\u3088\u3057\u30c1\u30fc\u30e0\u3067\u6771\u4eac\u30c9\u30fc\u30e0\u306e\u3042\u305f\u308a\u306e\u30ec\u30b9\u30c8\u30e9\u30f3\u3078\u884c\u304d\u3001\u30a2\u30e1\u30ea\u30ab\u30f3\u306a\u611f\u3058\u306e\u4f53\u306b\u60aa\u305d\u3046\u306a\u63da\u3052\u7269\u3044\u308d\u3044\u308d\u3092\u98df\u3079\u3066\u304d\u307e\u3057\u305f\u3002
\n\u80c3\u306e\u85ac\u98f2\u3093\u3067\u5bdd\u308b\u304b\u306a\u30fc\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"
\u53bb\u5e74\u50d5\u304c\u5199\u771f\u3092\u64ae\u308a\u307e\u304f\u3063\u3066\u3044\u305f\u304b\u3089\u304b (\u7b11)\u3001\u64ae\u5f71\u3057\u3061\u3083\u3060\u3081\u3088\u3001\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u3063\u3066\u3044\u305f\u306e\u3067\u3001\u4eca\u5e74\u306f\u6587\u7ae0\u3060\u3051\u3002 \u4eca\u65e5\u304b\u3089\u4e2d\u592e\u5927 @ \u5f8c\u697d\u5712 \u3067\u3084\u3063\u3066\u307e\u3059\u3002 \u521d\u65e5\u306e\u4eca\u65e5\u306f\u3001\u5927\u5b66\u3067\u3042\u308c\u3053\u308c\u3084\u3063\u3066\u305f\u306e\u3067\u3001\u5915\u65b9\u306e\u30dd\u30b9\u30bf\u30fc\u30bb\u30c3\u30b7\u30e7\u30f3 … “\u30b2\u30ce\u30e0\u5fae\u751f\u7269\u5b66\u4f1a” \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"activitypub_content_warning":"","activitypub_content_visibility":"","activitypub_max_image_attachments":4,"activitypub_interaction_policy_quote":"anyone","activitypub_status":"","footnotes":""},"categories":[10,4],"tags":[],"class_list":["post-2276","post","type-post","status-publish","format-standard","hentry","category-conference-logs","category-4"],"_links":{"self":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2276","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/comments?post=2276"}],"version-history":[{"count":0,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2276\/revisions"}],"wp:attachment":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/media?parent=2276"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/categories?post=2276"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/tags?post=2276"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}