{"id":1724,"date":"2007-07-11T11:07:28","date_gmt":"2007-07-11T02:07:28","guid":{"rendered":"http:\/\/yasu2.prosou.nu\/blog\/index.php\/2007\/07\/11\/uk-japan_systems_biology_works_1\/"},"modified":"2007-07-11T11:07:28","modified_gmt":"2007-07-11T02:07:28","slug":"uk-japan_systems_biology_works_1","status":"publish","type":"post","link":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/2007\/07\/11\/1724\/","title":{"rendered":"UK-Japan Systems Biology Workshop @ British Embassy, Tokyo"},"content":{"rendered":"<p>Every object that biology studies is a system of systems. (Frabcius Jacob: 1974)<br \/>\nTo understand just on life, you have to swallow the world (someone, 1981)<br \/>\n[ Genome-scale metabolic model of Mycobacterium tuberculosis: what does the TB bacillus eat? ]<br \/>\n\u6cbb\u7652\u306b\u306f6\u30f6\u6708\u304b\u304b\u308b\u3002slow growth mechanism \u3092\u3076\u3063\u58ca\u3057\u305f\u3044\u3002<br \/>\n\u6025\u901f\u306b\u767a\u75c7\u3059\u308b\u306e\u306f\u307b\u3068\u3093\u3069\u304c\u5b50\u4f9b\u3078\u306e\u611f\u67d3\u306e\u5834\u5408\u3002<br \/>\n5-10% \u304c\u3059\u3050\u767a\u75c7\u3057\u300190-95% \u304c\u6f5c\u4f0f\u3002<br \/>\n4 drugs for 6 months to kill TB. \u3067\u3082\u3044\u308d\u3044\u308d\u6bba\u3057\u3061\u3083\u3046\u3002<br \/>\n\u526f\u4f5c\u7528\u3092\u56de\u907f\u3059\u308b\u305f\u3081\u306b\u306f\u3082\u3063\u3068\u77ed\u671f\u9593\u3067\u6e08\u3080\u85ac\u5264\u304c\u5fc5\u8981\u3002<br \/>\nchemostat \u3092\u4f7f\u3063\u3066\u3001in vivo \u3067 TB \u3092\u3044\u304f\u3064\u304b\u306e\u72b6\u614b\u3067\u5b9a\u5e38\u72b6\u614b\u306b\u6301\u3063\u3066\u3044\u304d\u3001transcriptome \u306a\u3069\u3092\u3068\u308b\u3002<br \/>\nFBA \u3067\u30e2\u30c7\u30eb\u3092\u7acb\u3066\u3066\u308b\u2192mutation \u306e\u7d50\u679c\u3092\u4e88\u6e2c\u3059\u308b\u306e\u306b\u3082\u4f7f\u3048\u308b\u305c\u30fc\u3002<br \/>\n\u3057\u304b\u3057\u3001pathway \u304c\u3044\u308d\u3044\u308d\u308f\u304b\u3089\u306a\u304b\u3063\u305f\u308a\u3068\u304b\u3001\u3044\u308d\u3093\u306a\u554f\u984c\u304c\u3042\u308b&#8230;<br \/>\n\u3069\u306e\u907a\u4f1d\u5b50\u304c essential \u304b\u3092 in vitro \u3067\u691c\u8a3c<br \/>\nTP 146 FP 87 TN 399 FN 85. \u3051\u3063\u3053\u3046\u3044\u3044\u611f\u3058\u3060\u3002<br \/>\nglycerol \u306e\u4f9b\u7d66\u3092\u6e1b\u3089\u3057\u3066\u3084\u308b\u3068\u3001\u500d\u5897\u306b\u304b\u304b\u308b\u6642\u9593\u304c19\u6642\u9593\u304b\u308969\u6642\u9593\u306b\u3002<br \/>\nTB \u3092\u306f\u3058\u3081\u3068\u3057\u3066\u3001\u591a\u304f\u306e\u6297\u539f\u304c\u5bbf\u4e3b\u5185\u3067\u9577\u751f\u304d\u3059\u308b\u305f\u3081\u306b Isocitrate lyase \u3092\u5fc5\u8981\u3068\u3059\u308b\u3002\u3053\u3044\u3064\u3092 knockout \u3057\u3066\u3084\u308b\u3068\u3001slow growth \u3067\u304d\u306a\u304f\u306a\u308b\u3053\u3068\u3092\u5b9f\u9a13\u3067\u78ba\u8a8d\u3057\u305f\u3002<br \/>\nGSMN-TB web server (Genome Biol. 2007, 8(5):R89)<br \/>\nfuture work: host metabolism \u3092\u5165\u308c\u3066\u3084\u308b\u5fc5\u8981\u3042\u308a (macrophage \u3068\u304b)<br \/>\n[ In vivo robustness analysis of cell division cycle in yeast with genetic Tug-Of-War method ]<br \/>\n\u3082\u308a\u3084\u3055\u3093 @ JST PRESTO \/ Cancer institute<br \/>\n&#8211; what&#8217;s gTOW ?<br \/>\n&#8211; ongoing projects with gTOW<br \/>\nKO (knockout) = reduce gene expression level to 0<br \/>\nKD (knockdown) = reduce to unknown level<br \/>\nOP (overexpression by promoter swapping) = increase to unknown level<br \/>\ngTOW = increase to some regulated level<br \/>\ntarget gene \u3092\u5165\u308c\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f5c\u3063\u3066\u3001\u305a\u304c\u30fc\u3063\u3068\u5897\u3084\u3059\u3093\u3060\u3051\u3069\u3001\u30b3\u30d4\u30fc\u6570\u3092\u5236\u5fa1\u3059\u308b\u305f\u3081\u306b bias \u304c\u304b\u3051\u3089\u308c\u308b\u3088\u3046\u306a\u8a2d\u8a08\u306b\u3057\u3066\u304a\u304f\u3002\u30b3\u30d4\u30fc\u6570\u306e\u5236\u5fa1\u304c\u3067\u304d\u308b\u3053\u3068\u306f GFP \u3092\u4f7f\u3063\u3066\u78ba\u8a8d\u3057\u3066\u308b\u3002<br \/>\n\u51fa\u82bd\u9175\u6bcd\u3067\u306f\u8a08\u7b97\u6a5f\u30e2\u30c7\u30eb\u3088\u308a\u3001\u5b9f\u969b\u306e\u7d30\u80de\u306e\u307b\u3046\u304c robust \u3060\u3063\u305f\uff01<br \/>\n\u5206\u88c2\u9175\u6bcd\u3067\u306f\u308f\u308a\u3068\u540c\u3058\u611f\u3058\u3002<br \/>\n\u4ed6\u306e\u5fae\u751f\u7269\u306b\u306f\u9069\u7528\u3067\u304d\u308b\uff1f<br \/>\n\u2192 i&#8217;m planning to apply on E.coli<br \/>\n[ The interplay between gene regulatory and metabolic reaction networks in streptomyces ]<br \/>\n\u74b0\u5883\u306b\u3088\u308b phenotypic switching<br \/>\nanalysis of microarray data in the context of constraint based models of genome scale metabolic reaction networks.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Every object that biology studies is a system of systems. (Frabcius Jacob: 1974) To understand just on life, y &hellip; <a href=\"https:\/\/yasu2.prosou.nu\/blog\/index.php\/2007\/07\/11\/1724\/\" class=\"more-link\"><span class=\"screen-reader-text\">&#8220;UK-Japan Systems Biology Workshop @ British Embassy, Tokyo&#8221; \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"activitypub_content_warning":"","activitypub_content_visibility":"","activitypub_max_image_attachments":4,"activitypub_interaction_policy_quote":"anyone","activitypub_status":"","footnotes":""},"categories":[10],"tags":[],"class_list":["post-1724","post","type-post","status-publish","format-standard","hentry","category-conference-logs"],"_links":{"self":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/1724","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/comments?post=1724"}],"version-history":[{"count":0,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/posts\/1724\/revisions"}],"wp:attachment":[{"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/media?parent=1724"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/categories?post=1724"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/yasu2.prosou.nu\/blog\/index.php\/wp-json\/wp\/v2\/tags?post=1724"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}